METHOD AND SYSTEM FOR CLASSIFICATION AND QUANTITATIVE ANALYSIS OF CELL TYPES IN MICROSCOPY IMAGES

摘要

The disclosed embodiments are directed to a method for accurately counting and characterizing multiple cell phenotypes and sub-phenotypes within cell populations simultaneously by exploiting biomarker co-expression levels within cells of different phenotypes in the same tissue sample. The disclosed embodiments are also directed to a simple intuitive interface enabling medical staff (e.g., pathologists, biologists) to annotate and evaluate different cell phenotypes used in the algorithm and the presented through the interface.

主权项

1. A method of quantifying cells of a particular cell type in a sample using a bio-semantic model comprising:
collecting of images from a plurality of biomarkers from different fields of views, the plurality of biomarker images comprising at least images of two biomarkers present in the said cell type, wherein at least one is cell-type specific, and at least one segmented image per field of view at the single cell level; annotating a sub-set of cells for being positive or negative for a particular biomarker on the representative images of the signal distribution of the biomarker; and building a classification algorithm applying a partially supervised multi-class, multi-label hierarchical cell classification based on a bio-semantic model to determine quantity of a plurality of the cell-type specific cells in the biological sample.