AU NANOPARTICLES ENCAPSULATED IN NANOCOMPOITES AND APPLICATIONS THEREOF IN RAPID DETECTION OF AN ANALYTE

摘要

A method of preparation for a detection pad for detecting an analyte. The method includes preparing a labeling substance including a plurality of metal nanoparticles encapsulated in a nanocomposite complex. The labeling substance may be conjugated with at least one analyte-binding partner to prepare a labeled detection reagent. The detection reagent may then be applied to a pad to prepare a reagent absorption pad. A control pad may then be prepared, and finally the control pad and conjugation pad may be bound together to form the detection pad.

主权项

1- A method of preparing a detection pad, comprising the steps of:
preparing a labeling substance, wherein the labeling substance comprises a plurality of metal nanoparticles encapsulated in a nanocomposite complex; conjugating the labeling substance with at least one analyte-binding partner to prepare a labeled detection reagent; applying the prepared detection reagent into a pad to prepare a reagent absorption pad; preparing a control pad, wherein the control pad comprises of a nitrocellulose strip pad comprising a test zone and a control zone; and binding the control pad and conjugation pad to obtain the detection pad.