GAMMA STERILIZED DEXTRAN SOLUTIONS AND METHODS OF USE

摘要

Provided herein are kit for providing a gamma sterilized aqueous dextran solution that increase the efficiency of blood separation by allowing the dextran solution to be sterilized by exposure to gamma radiation while maintaining sufficient molecular weight to act as a red blood cell aggregate. Also provided are methods of use.

主权项

1. A kit for providing a gamma sterilized aqueous dextran solution for red blood cell aggregation comprising:
solutes, the solutes comprising;
dextran, where the dextran has an initial average molecular weight greater than 500 kD; and2.0 to 20.0 wt % ascorbic acid or its mineral salt to the dextran; a mixing vessel for containing and enabling a sample comprising red blood cells (RBC) to separate into two or more distinct layers of sub materials, said mixing vessel having two or more valve ports positions for introducing and extracting materials; a receptacle in fluid communication with the mixing vessel through one of the valve ports configured to contain a 1 to 10 wt/v % of an aqueous solution of the solutes; and where the kit is capable of undergoing gamma sterilization upon exposure to gamma irradiation at a dose between 20 and 50 kGy such that the aqueous solution, when contained in the receptacle, has an average molecular weight greater than 200 kD after gamma irradiation.