Deimmunized Serum-Binding Domains and Their Use in Extending Serum Half-Life

摘要

The present invention is directed to a polypeptide (for example, an antigen-binding molecule) that comprises a polypeptide portion of a deimmunized serum-binding protein capable of binding to said serum protein. The presence of the serum-binding protein extends the serum half-life of the polypeptide, relative to the serum half-life of the polypeptide if lacking the polypeptide portion of the deimmunized serum-binding protein. The invention also pertains to methods and uses that employ such molecules.

主权项

1. A polypeptide that comprises a portion of a deimmunized albumin-binding protein capable of binding to serum albumin; wherein said deimmunized albumin-binding protein portion is a variant of a wild-type albumin-binding domain (ABD) of a Streptococcal Protein G, said wild-type ABD having the amino acid sequence of SEQ ID NO:304; wherein said variant ABD has an amino acid sequence that differs from that of SEQ ID NO:304 in comprising:
(A) a tyrosine to alanine variation at position 21 of SEQ ID NO:304 and an isoleucine to alanine variation at position 25 of SEQ ID NO:304; or (B) an asparagine to serine variation at position 26 of SEQ ID NO:304 and a threonine to serine variation at position 30 of SEQ ID NO:304; wherein said deimmunized albumin-binding protein portion extends the serum half-life of said polypeptide, relative to the serum half-life of said polypeptide if lacking said portion of said deimmunized albumin-binding protein.