METHODS AND COMPOSITIONS FOR ENHANCED CELLULAR SELECTIVITY USING NANOCARRIER-ASSOCIATED LIGANDS

摘要

The presently disclosed subject matter provides methods and compositions for activation of clustered receptors on a target cell using nanocarrier-associated ligands, particularly methods and compositions for targeted activation of clustered receptors on antigen-experienced T cells using nanocarrier-associated antibodies.

主权项

1. A method for selectively enhancing activation of antigen-experienced cells, the method comprising:
a) administering a selected antigen to target cells in an amount sufficient to produce microclustering of ligand-binding receptors on the surface of the target cells, thereby producing antigen-experienced cells comprising microclustered ligand-binding receptors; and b) administering nanocarrier-associated ligands and the selected antigen to a plurality of cells, wherein the plurality of cells comprises antigen-experienced cells and naïve cells;wherein the nanocarrier-associated ligands bind microclustered ligand-binding receptors of the antigen-experienced cells, thereby selectively enhancing activation of the antigen-experienced cells as compared to:
i) the binding of free ligands to the microclustered ligand-binding receptors; and ii) the binding of nanocarrier-associated ligands to ligand-binding receptors of naïve cells, wherein the naïve cells are non-specifically activated.