Fluorescence spectroscopy in absorbing media

摘要

Procedure for the detection of an analyte in a sample by fluorescence measurement, comprises providing a detection medium containing the sample, optionally at least an analyte detecting reagent, a fluorophore or its preliminary component, an absorber that absorbs light over a part of the excitation range of the fluorophore; irradiating the medium with light for the excitation of the fluorophore within the range of the second wavelength; and determining the fluorescence emission of the fluorophore in one or more measuring wavelengths. Procedure for the detection of an analyte in a sample by fluorescence measurement, comprises providing a detection medium containing at least a part of the sample, in which the analyte has to be detected, optionally at least a reagent for the detection of the analyte, a fluorophore that possesses an excitation range with at least an excitation maximum with a first wavelength, or a fluorophore preliminary component, from which the fluorophore is formed in the presence of the sample and optionally the reagent, an absorber that absorbs light over a part of the excitation range of the fluorophore; irradiating the medium with light for the excitation of the fluorophore within the range of the second wavelength; and determining the fluorescence emission of the fluorophore in one or more measuring wavelengths to determine the quantity or activity of the analyte in the sample. Independent claims are included for: (1) a test element, for the detection of an analyte, comprising the fluorophore, absorber and optionally at least a reagent for the detection of an analyte, where the fluorophore and/or fluorophore preliminary component and absorber are located on the test element in such a manner that the irradiated light for the excitation of the fluorophore comes in contact at first with the absorber and then with the fluorophore or meets both the absorber and the fluorophore simultaneously, and the system from fluorophore and absorber results in an effectively changed excitation maximum with a second wavelength that is different from the first; and (2) use of a test element in a procedure, for the detection of an analyte in a sample, comprising contacting the test element with the sample, irradiating the sample with a light for the excitation of the fluorophore within the range of the second wavelength, and determining the fluorescence emission of the fluorophore.