Recombinant MVA viruses expressing clade A/G, clade B, and clade C modified HIV env, gag and pol genes

摘要

The invention provides modified vaccinia Ankara (MVA), a replication-deficient strain of vaccinia virus, expressing human immunodeficiency virus (HIV) env, gag, and pol genes.

主权项

1. A pharmaceutical composition comprising a recombinant MVA virus comprising a sequence encoding an HIV Env antigen inserted into deletion site II of an MVA and a sequence encoding HIV Gag and Pol antigens inserted into deletion site III of the MVA genome, and a pharmaceutically acceptable carrier;
wherein the sequence encoding the HIV Env antigen comprises SEQ ID NO:3 and the sequence encoding the HIV Gag and Pol antigens comprises SEQ ID NO: 4; wherein the transcription of the sequence encoding the HIV Env antigen is under the control of a first promoter and transcription of the sequence encoding the HIV Gag and Pol antigens is under the control of a second promoter and wherein the noncoding sequence between the first promoter and the initiation codon of the sequence encoding the HIV Env antigen comprises SEQ ID NO:8.