| 摘要 |
A device and method for extracting nucleic acids, comprising a hollow body, preferably a pipette tip, through which a fluid is guided, characterized in that a material with a rough or structured surface is arranged in this hollow body such that the material can be washed around with a fluid. After lysis of the sample and adjusting necessary binding conditions for the adsorption of the nucleic acids on the carrier material, the formulation is "pipetted past" the nucleic acid binding material located vertically in the pipette tip multiple times by means of a pipette process. The nucleic acids bind to the material. Subsequently, washing buffers are also "pipetted past" the nucleic acid binding material. A drying step subsequently takes place. Finally, the eluent is in turn "pipetted past" the nucleic acid binding material multiple times, said material being arranged vertically, and the bound nucleic acid is thereby dissolved. The nucleic acid is now available for necessary downstream application. |
