DETECTION OF BACTERIUM BELONGING TO GENUS CAMPYLOBACTER WHICH TARGETS CYTOLETHAL DISTENDING TOXIN

摘要

Disclosed are: a cytolethal distending toxin (CDT) derived from C. hyointestinalis; a polynucleotide encoding the cytolethal distending toxin; and a novel method for detecting C. hyointestinalis by using cdt gene. Attention is focused on a cytolethal distending toxin (CDT) derived from a bacterium belonging to the genus Campylobacter. As a result, a cell line is found whose cdtB gene cannot be amplified by a multiplex PCR method that can detect specifically cdtA gene, cdtB gene and cdtC gene of C. jejuni, C. coli and C. fetus but can be amplified with a primer common to all of these three cell lines,by detecting cdt gene from a bacterium belonging to the genus Campylobacter isolated from a Thai enteritis patient. The analysis is made on 16S rRNA gene, and the cell line is identified as C. hyointestinalis. The full-length nucleotide sequence for cdt gene is determined by the genome walking on the upstream and the downstream from cdtB gene.