Method for of identification of Gram-negative bacteria using the MALDI-TOF MS method

摘要

The present invention relates to the identification of lipopolysaccharide chemotypes of Gram-negative bacteria based on the endotoxin profile of whole bacteria using MALDI-TOF MS. The profile derived from lipopolysaccharides (LPS) for bacterial preparation is obtained in the negative mode of a MALDI-TOF spectrometer using DHB as a matrix. The subject invention is useful in the analysis of clinical samples, diagnosis of infections caused by Gram-negative bacteria and can be useful as a tool in the therapy directed against the O-antigen using specific antibodies.