| 发明名称 |
METHODS AND COMPOSITIONS FOR RNA-GUIDED TREATMENT OF HIV INFECTION |
| 摘要 |
A method of inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus by treating the host cell with a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and two or more different guide RNAs (gRNAs), wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) in the proviral DNA, and inactivating the proviral DNA. A composition for use in inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus including isolated nucleic acid sequences comprising a CRISPR-associated endonuclease and a guide RNA, wherein the guide RNA is complementary to a target sequence in a human immunodeficiency virus. |
| 申请公布号 |
US2016250300(A1) |
申请公布日期 |
2016.09.01 |
| 申请号 |
US201615148261 |
申请日期 |
2016.05.06 |
| 申请人 |
TEMPLE UNIVERSITY OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION |
发明人 |
KHALILI Kamel;HU Wenhui |
| 分类号 |
A61K38/46 |
主分类号 |
A61K38/46 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus, including the steps of:
treating the host cell with a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and two or more different guide RNAs (gRNAs), wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) of the proviral DNA; and inactivating the proviral DNA. |
| 地址 |
Philadelphia PA US |
