METHODS FOR OBTAINING OIL FROM MAIZE USING ACID PROTEASE AND CELL-WALL POLYSACCHARIDE-DEGRADING ENZYMES

摘要

Disclosed are methods for obtaining oil from maize, involving grinding maize kernels to form flour, adding water to the flour to form a slurry, and incubating the slurry with α-amylase for about 10 minutes to about 180 minutes at a temperature of about 75° to about 120° C. and at a pH of about 3 to about 7 to form a mash, cooling the mash to about 15° C. to about 40° C. and adding a nitrogen source, glucoamylase, yeast, acid protease, and cell-wall polysaccharide-degrading enzymes to form a beer containing ethanol and oil, wherein the beer has a pH of about 3 to about 7, and recovering oil from the beer.