| 摘要 |
FIELD: biotechnology.SUBSTANCE: invention relates to biochemistry, genetic engineering and biotechnology, namely to strain Escherichia coli BL21(DE3)GoldpETCYPopti. This strain is producer of recombinant human cyclophilin A. Strain is produced by cell transformation of strain Escherichia coli BL21(DE3)Gold by pETCYPopti plasmid. Said plasmid has size of 5858 base pairs and contains fragment XhoI-NdeI of vector pET-22b(+) carrying gene of ampicillin resistance ampR, promoter and terminator of phage T7 RNA-polymerase and polylinker, wherein fragment, carrying gene of human recombinant cyclophilin A, is cloned at sites XhoI-NdeI with size of 498 base pairs, SEQ ID NO: 1.EFFECT: present invention enables to obtain human cyclophilin A with high output and purity.1 cl, 3 dwg, 1 ex |
