| 摘要 |
Kinase and nucleotidyltransferase enzymes for the production of activated sugars have been developed. These enzymes have improved stability for industrial application and relaxed specificity towards a variety of sugars. These enzymes are useful in, for example, the production of diverse NDP-sugars for glycosylation of aglycones of interest, production of oligosaccharides, production of other important glycoylated sugars, and in drug discovery applications. |
| 主权项 |
1. An isolated, mutant sugar-1-kinase, wherein the isolated sugar-1-kinase has sugar-1-kinase activity in a sugar-1-kinase assay, and wherein the sugar-1-kinase comprises SEQ ID NO:10 and further comprises the following mutations:
(i) I312T, L332H, Y341P or Y341M, and F342K or F342T; (ii) I312T and L332H; (iii) Y341P or Y341M and F342K or F342T; (iv) I312T, L332H, and Y341P or Y341M; or (v) I312T, L332H, and F342K or F342T. |
