Liquid chromatography and apparatus for the same

摘要

A definite, but large amount of a sample solution is fed to a small concentration column filled with a packing material to retain in the concentration column components to be measured in the sample solution. Then, a small amount of a desorbing solution is fed to the concentration column to desorb the components retained in the concentration column. The effluent solution containing the desorbed components is fed to a separation column filled with a packing material. An eluting solution is fed to the separation column to separate the components from one another, and the components are detected by a detector.