| 发明名称 | DNA polymerases | ||
| 摘要 | The invention provides novel mutant thermostable DNA polymerases having desirable properties such as increased polymerase efficiency and speed, increased affinity to DNA substrates and/or increased resistance to DNA polymerase inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel DNA polymerases. | ||
| 申请公布号 | US9493848(B2) | 申请公布日期 | 2016.11.15 |
| 申请号 | US201213564940 | 申请日期 | 2012.08.02 |
| 申请人 | Thermo Fisher Scientific Baltics UAB | 发明人 | Skirgaila Remigijus;Tubelevi{hacek over (c)}iūté Agne;Rim{hacek over (s)}eliėnė Renata;Burinskas Sigitas |
| 分类号 | C12N9/12 | 主分类号 | C12N9/12 |
| 代理机构 | McNeill Baur PLLC | 代理人 | McNeill Baur PLLC |
| 主权项 | 1. A DNA polymerase mutant comprising SEQ ID NO: 13 with a mutation at position E189 and optionally one or more of the following mutations: E230K, E76G, E76K, E76A, E90K, D551N, K206R, Q680R, L552R, H28R, E315K, L30R, L30P, D452N, G504S, G504D, G38R, F73V, H75R, A348V, L351F, A439T, L552R, I553V, D578N, H676R, D732G, E734G, E734K, F749V, E507K, E507A; wherein the DNA polymerase mutant exhibits relative to wild-type DNA polymerase of SEQ ID NO: 13, and under the same conditions, increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor. | ||
| 地址 | Vilnius LT | ||