| 发明名称 |
Artificial introns |
| 摘要 |
The invention concerns the field of recombinant gene engineering. It concerns novel artificial introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel artificial intron sequences. |
| 申请公布号 |
US9493803(B2) |
申请公布日期 |
2016.11.15 |
| 申请号 |
US201314134177 |
申请日期 |
2013.12.19 |
| 申请人 |
Boehringer Ingelheim International GmbH |
发明人 |
Enenkel Barbara |
| 分类号 |
C12N15/113;C12P21/00;C07K16/00;C12N15/67;C12N15/85;C12N15/13;C07H21/04;C12P21/02 |
主分类号 |
C12N15/113 |
| 代理机构 |
|
代理人 |
Morris Michael P.;Lebel Edouard G.;Royaee Atabak R. |
| 主权项 |
1. A transcription unit or expression vector comprising a gene of interest and comprising at least one artificial intron, whereby the artificial intron is
i. replacing a native intron sequence within said gene of interest, or ii. positioned at the site of a native intron position within said gene of interest, wherein the gene of interest is an immunoglobulin gene. |
| 地址 |
Ingelheim am Rhein DE |
