| 摘要 |
Method and arrangement for detecting binding events of molecules, in which, to at least one first molecule which is immobilised on a bioactive surface by a covalent bond, at least one second molecule has been bonded or is bonded, with formation of at least one specific non-covalent bond, the second molecule being bonded or having been bonded chemically covalently to a ligand. The bioactive surface can be contacted with at least one analyte molecule, binding events between the analyte molecule and the ligand being able to be detected by means of an analytical measuring method. The at least one specific non-covalent bond between the first and second molecule is breakable again by supplying a buffer solution. According to the invention, a third molecule is bonded to the second molecule, the third molecule also having a ligand. A specific ligand, or two specific ligands which are different from each other, can hence be available on the bioactive surface in an easily reversible manner, |
