IMMUNE MONITORING TO PREDICT AND PREVENT INFECTION

摘要

Methods of evaluating the risk of infection after transplantation or immunosuppression in a subject are provided. Methods of determining whether a subject should be treated prophylactically with an anti-microbial agent are also provided herein. Kits for performing the methods described herein are also provided.

主权项

1. A method of evaluating the risk of infection after immunosuppression in a subject comprising: (a) obtaining a sample containing T cells from the subject; (b) stimulating T cells obtained from the subject with an antigen in vitro; (c) determining expression levels of at least three markers selected from CD107, IFN-γ, IL-2 and TNF-α in response to the antigen stimulation of the T cells; and (d) evaluating the risk of infection in the subject based on the expression of at least three markers selected from CD107, IFN-γ, IL-2 and TNF-α in each of the T cells, wherein when the number of T cells expressing at least three of the markers is higher than in controls, the subject is at high risk of infection.