Microscope and ghosting elimination method

摘要

A microscope includes a first imaging optical system that images sample transmitted light transmitted through a sample provided on a stage, and a second imaging optical system that images a part of the sample transmitted light branched from the first imaging optical system. Here, the second imaging optical system includes a light beam branching element that branches the part of the sample transmitted light from the first imaging optical system, and has a thickness of a predetermined threshold or more, an imaging element that images a phase difference image of the branched sample transmitted light, one or a plurality of optical elements that images an image of the phase difference image of the branched sample transmitted light on the imaging element, and a filter that shields a part of the branched sample transmitted light imaged on the imaging element.

主权项

1. A microscope, comprising:
an objective lens that receives light transmitted through a sample provided on a stage and converges the light; a first imaging optical system configured to image the convergent light; and a second imaging optical system configured to image a part of the convergent light branched from the first imaging optical system, wherein the second imaging optical system includes
a light beam branching element that branches part of the convergent light that is still converging when it reaches the light beam branching element, and has a thickness of a predetermined threshold or more,an imaging element that images a phase difference image of the branched light,at least one optical element positioned to receive the convergent light from the light beam branching element, and to direct the light to the imaging element, where the convergent light is still converging when it reaches the at least one optical element, anda filter that shields a part of the branched sample transmitted light imaged on the imaging element, wherein the filter is a diaphragm in which a through hole set for allowing a luminous flux set which becomes the phase difference image to pass therethrough is provided, and wherein the thickness of the light beam branching element has a larger value than a feature value calculated based on a diameter of the through hole, a center distance between the through holes of the through hole set, and a luminous flux diameter at a position of the filter of the sample transmitted light.