Cell cultivation in chitosan alginate hydrogel beads

摘要

The present invention relates to a hydrogel matrix comprising cartilage-forming cells wherein alginate, chitosan and cartilage-forming cells are mixed and subsequently polymerized into beads.

主权项

1. A spherical hydrogel bead with a diameter between 0.01 and 5 mm, comprising a homogeneous matrix of alginate and native chitosan of Mw lower than 60 kD and further comprising cartilage-forming cells within said matrix, characterised in that said bead comprises between 1% and 1.4% (w/v) of alginate and between 0.5% and 0.7% (w/v) of native chitosan, wherein the hydrogel beads comprise inter-connected chitosan trabeculae within an alginate gel.