PCV2 ORF2 protein variant and virus like particles composed thereof

摘要

Vaccination methods to control PCV2 infection with different PCV2 subtypes are disclosed. Specifically, a PCV2 subtype b (PCV2b) ORF2 proteins or immunogenic compositions comprising a PCV2b ORF2 protein are used in a method for the treatment or prevention of an infection with PCV2 of the same PCV2b and/or different subtype; the reduction, prevention or treatment of clinical signs caused by an infection with PCV2 of the same PCV2b or a different subtype; and/or the prevention or treatment of a disease caused by an infection with PCV2 of the same PCV2b and/or a different subtype. The present invention in particular relates to PCV2 subtype b (PCV2b) ORF2 proteins characterized in that they contain at least one mutation in the BC loop that such that the expressed protein is preferably expressed in a higher amount compared to a PCV2 ORF2 protein that does not contain such mutation.

主权项

1. A or recombinant baculovirus vector plasmid a polynucleotide comprising a sequence which encodes a polypeptide consisting of the following (a) and (b):
(a) a PCV2 ORF2 protein having at least 90% sequence identity to SEQ ID NO:5 over the entire 233 amino acid length comprising:
at amino acid position 59 an arginine residue or a lysine residue, andat amino acid position 88 a proline residue, andat amino acid position 151 a threonine residue, andat amino acid position 206 an isoleucine residue, andat amino acid position 232 an asparagine residue,wherein the numbering of the amino acid positions refers to the amino acid sequence of wild type PCV2 ORF2 protein; and (b) the PCV2 ORF2 protein characterized in (a) and that it (i) contains at least one substitution mutation in the BC loop at amino acid position 63 wherein the at least one substitution is an amino acid residue other than an arginine residue or a lysine residue, and (ii) is expressed in a higher amount compared to an expressed PCV2 ORF2 protein that does not contain such mutation in the BC loop.