Plasminogen-activating substance and the preparation and use of such substance

摘要

A new type of plasminogen-activating substance is extracted from the bile of hogs, which substance is characterized by; (A) a molecular weight of 80,000 +/-10,000; (B) an isoelectric point of 7.1; (C) an ultraviolet absorption spectrum showing a peak ( lambda max) at a wave length of about 278 nm; (D) a fibrinolysis activity such that a Ca-added standard fibrin plate is lysed but a heated fibrin plate is not lysed; (E) such a high thermal stability that heating at a temperature of 80 DEG C. for 10 minutes causes the plasminogen-activating activity to be decreased to an extent corresponding to 50% of the original activity and heating at a temperature of 60 DEG C. for 30 minutes causes the plasminogen-activating activity to be decreased to an extent corresponding to from 30 to 40% of the original activity; (F) a solubility in a saline solution and an insolubility in water-soluble organic solvents, and; (G) a synthetic substrate-lysis activity such that Glutaryl-Gly-Arg-MCA and Pro-Phe-Arg-MCA are only marginally hydrolysed.