GLP-1 ANALOG FUSION PROTEIN AND PREPARATION METHOD AND USE THEREOF

摘要

The present invention provides a novel GLP-1 analogue fusion protein and a method for preparing the fusion protein. The fusion protein consists of three regions as follows: GLP-1 analogue-linker peptide-HSA (Human Serum Albumin). Compounds which contain GLP-1 analogues prepared by adopting the present invention have the advantages of very low production cost, higher biological activity and better in-vivo and in-vitro stability. The fusion protein can be used for treating diabetes, obesity, irritable bowel syndrome and other diseases which can be benefited by reducing plasma glucose, inhibiting stomach and/or intestine movement and inhibiting stomach and/or intestine emptying or inhibiting food intake.

主权项

1. A GLP-1 analogue fusion protein, characterized in that a structure of the fusion protein is GLP-1 analogue-linker peptide-human serum albumin, the length of the linker peptide does not exceed 26 amino acids and a general formula is (Xaa)x-(Pro)y-(Xaa)z, wherein Xaa is one or any combination of a plurality of A and S, x, y and z are integers, x, z≧3, 26≧x+y+z≧14, 10≧y≧3, 1≧y/(x+z)≧0.13, an N-terminal of the linker peptide is connected with a C-terminal of the GLP-1 analogue through a peptide bond, and a C-terminal of the linker peptide is connected with an N-terminal of the human serum albumin through a peptide bond.