| 摘要 |
<p>In a transverse phosphite process for preparation of polynucleotides, a 5" reactive resin nucloside is reacted with a bifunctional phosphorus (III) reagent to produce a monofunctional resin nucleosidophosphorus (III) intermediate. This intermediate is condensed with a 3" reactive 5" blocked nucleoside to produce a dinucleosidophosphite. The latter is 5" deblocked and the cycle of reaction with bifunctional intermediate and condensation with 5" blocked 3" reactive nucleoside repeated to build a chain corresponding to the desire polynucleotide. Phosphite is converted to phosphate by oxidation to produce the polynucleotide. Improved structures and processes for linking the nucleotide chain to the resin, and improve techniques for detaching the final nucleotide from the resin are also disclosed. </p> |
