| 摘要 |
PURPOSE:An electrophoretically separated protein is activated with peroxidase to develop its color with hydrogen peroxide, then the excessive hydrogen peroxide is decomposed with a catalase to stop staining whereby protein spots are detected in stabilized states. CONSTITUTION:Proteins on a support are subjected to electrophoresis to separate into individuals, then dipped in, as such, in a hemin solution so that the proteins absorb hemin and become active to peroxidase. Then, the support is dipped in a solution of pigment precursor and the protein spots are stained with hydrogen peroxide. Then, the excessive amount of hydrogen peroxide is decomposed with catalase to stop staining on the protein spots. USE:Inspections of medical specimens.
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