| 摘要 |
<p>The invention relates to a process for the obtention of a glycosphingolipids complex characterized by the preparation in the first step of an acetone-dried powder of animal brains by means of their anhydrification through successive acetone treatments; then, from the prepared powder, gangliosides are axtracted with a solvent solution (water/methanol/chloroform) in a way that they remain dissolved in the aqueous phase impurified with phospholipids; then a purification is performed by Silica gel columm chromatography changing the polarity of eluent from an apolar solvent mixture that eluates phospholipids, remaining the gangliosides in the columm, to a more polar one that eluates gangliosides. Once the gangliosides are obtained, these are fractionated through ionic-exchange columm chromatography. Finally, ganglioside mixture is reformulated in order to obtain the desired glycosphingolipid complex.</p> |
