发明名称 |
AV NUKLEOSIDITRIFOSFAT BEROENDE 1-METYLHYDANTOINAS OCH DESS ANVAENDNING. |
摘要 |
The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions. The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it. Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination (a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or (b) of the simultaneously formed nucleoside diphosphate. |
申请公布号 |
FI850732(L) |
申请公布日期 |
1985.08.25 |
申请号 |
FI19850000732 |
申请日期 |
1985.02.22 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
SIEDEL, JOACHIM;DEEG, ROLF;ROEDER, ALBERT;ZIEGENHORN, JOACHIM;MOELLERING, HANS;GAUHL, HELMGARD |
分类号 |
C12N9/14;C12N9/86;C12Q1/34;C12R1/01;C12R1/06;C12R1/13;C12R1/265;G01N33/70 |
主分类号 |
C12N9/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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