| 摘要 |
PURPOSE:To separate a large amount of an oligosaccharide in high purity, by treating the oligosaccharide by column chromatography using a synthetic adsorbent free from ion exchange group as the filler. CONSTITUTION:A sugar syrup composed of e.g. an oligosaccharide having a glucose polymerization degree of 1-10 (G1-10) is adjusted to a concentration of preferably 5-15%, and passed through a column packed densely with a synthetic adsorbent having preferably fine and uniform particle size, free from ion exchange group, and substituted with pure water. The amount of the sugar syrup to be passed through the column is preferably 5-10vol% of the amount of the adsorbent. Thereafter, water or an alcohol (e.g. ethanol) having a proper concentration is passed through the column to elute the adsorbed oligosaccharide successively from the one having lower polymerization degree.
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