| 摘要 |
PURPOSE:To decrease noise and drift without entailing a decrease in sensitivity and an increase in holding time by using an arom. base acid having high anion eluting power as a UV absorbent eluting agent in place of a phthalic acid. CONSTITUTION:The arom. tribasic acid as the UV absorbent eluting agent is added into a polar mobile phase and the sepn. of anions is executed. The anions are detected from the UV absorbancy of the separated effluent by the arom. Tribasic acid. More specifically, the arom. tribasic acid is used in place of the conventional UV absorbent eluting ion in reversed-phase chromatography. The arom. tribasic acid is exemplified by benzene tricarboxylic acids such as trimesic acid, trimellitic acid and hemellitic acid. The time for analysis is thereby improved as compared to conventional practice and since the background can be decreased, the improvement of the noise, drift, sensitivity and linearity is possible.
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