| 摘要 |
The present invention provides a novel apparatus for culturing mammalian cells, whereby a plastic housing encasing a double membrane bag or bags supplies nutrients and oxygen to mammalian cells for propagation thereof. The double membrane bag contains a first bag formed of a microfiltration or ultrafiltration membrane disposed within a second membrane bag formed of a liquid impermeable, gas permeable membrane. This apparatus has a first chamber containing oxygen, a second chamber containing cells and a third chamber containing nutrients.
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