发明名称 |
Immobilization of nucleic acids in solvolyzed nylon supports. |
摘要 |
<p>Immobilization of nucleic acids, e.g., DNA and RNA, by contact with a solid support comprising nylon whose amide groups have been partially solvolyzed. Solvolysis of the nylon support can be accomplished by treatment with an alkylating agent such as a trialkyloxonium salt under anhydrous conditions followed by addition of water. The immobilized nucleic acid is particularly useful as an immobilized probe in hybridization assays to detect specific polynucleotide sequences in a test sample.</p> |
申请公布号 |
EP0219695(A1) |
申请公布日期 |
1987.04.29 |
申请号 |
EP19860112899 |
申请日期 |
1986.09.18 |
申请人 |
MILES LABORATORIES, INC. |
发明人 |
CARRICO, ROBERT J.;HATCH, ROBERT P.;PATTERSON, WILLIAM L. |
分类号 |
G01N33/53;C07H21/00;C07H21/04;C12Q1/68;G01N33/50;G01N33/545;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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