| 摘要 |
PURPOSE:To carry out the separation and purification of glutamine from an aqueous solution of glutamine containing impurities such as glutamic acid, pyrrolidonecarboxylic acid, sulfate radical, etc., by treating the solution with ion exclusion chromatography using a strongly acidic cation exchange resin. CONSTITUTION:Glutamine can be separated and purified from an aqueous solution of glutamine containing impurities composed mainly of glutamic acid, pyrrolidonecarboxylic acid, sulfate radical and pigment (e.g. glutamine fermentation liquid, dissolved liquid of crude glutamine crystal separated from the fermentation liquid, mother liquor of glutamine crystallization, etc.) by subjecting the solution to ion exclusion chromatography. The strongly acidic cation exchange resin used in the ion exclusion chromatography is preferably a styrene resin having a crosslinking degree of 4-8% to attain the highest separation performance. The operation temperature is selected below the heat-resistant temperature of the strongly acidic cation exchange resin.
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