Methods of immunoassay.

摘要

The present invention relates to dual analyte enzyme immunoassays for assaying two antigens in a single liquid sample wherein the two immunoreactions are carried out simultaneously and wherein subsequently the two enzyme reactions occur simultaneously. Suitable enzyme/substrate pairs are β-galactosidase/­nitrophenyl-β-D-galactosidase (p- and/or o-) and alkaline phosphatase/phenolphthalein monophosphate.