GLUCOSE-6-PHOSPHORIC ACID DEHYDROGENATED ENZYME LABELLED COMPOSITE AND ENZYME IMMUNOLOGICAL MEASUREMENT OF ERYTHROPOIETIN

摘要

PURPOSE:To enable highly sensitive measurement of erythropoietin (EPO) by coupling an immunochemically active substance having a thiol group in a molecule to a specified enzyme using a compound given by a specified formula to obtain a stable specified enzyme-labelled composite. CONSTITUTION:An immunocheimcally active substance having a thiol group in a molecule or bonded to a thiol group is coupled to a glucose-6-phosphoric dehydrogenated enzyme as label substance through a compound as given by the formula, wherein m represents an integer of 0-5, R, bivalent 6-member cyclic hydrogen carbon residual group and n, an integer of 0-1. Thus, an enzyme immunological measurement is done using a labelled composite in which erythropoietin or an antibody tended to react specifically with erythropoietin is coupled to a glucose-6-phosphoric acid dehydrogenated enzyme as label substance by a compound as given by the formula.