| 摘要 |
PURPOSE:To measure an acidic material to be measured with high accuracy by adding a positive charge material to a sample soln. contg. the acidic material to be measured and protein to form iron pairs and removing a protein component in a column. CONSTITUTION:The positive charge material is added to the sample soln. contg. the acidic material to be measured and the protein to form the ion pairs. The ion pairs are formed by adding alkylamine and tetraalkyl ammonium salt as the positive charge material to, for example, homovanillic acid, vanyllyl mandelic acid, etc., which are the acidic material to be measured. The sample soln. 1 contg. the ion pairs is passed through a selector valve 4 and the protein component is removed from an outlet F of the valve 4 without adsorbing the same in a protein removing column 5. An eluate 6 is then passed through the valve 4 and the adsorbed ion pairs are removed in the column 5 and are separated in a column 8, by which the acidic material to be measured is analyzed. Since the protein component is removed, the analysis of the acidic material to be measured with high accuracy without being affected by the protein component is permitted.
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