| 摘要 |
PURPOSE: To quickly separate and measure the binding species of a labeled reagent on a testing apparatus which does not require any cleaning process, etc., by performing the aliquot of a reaction mixture in the separation zone of the testing apparatus and, in addition, impressing a magnetic field upon the separation zone. CONSTITUTION: When the aliquot of a reaction mixture is performed in the separation zone of a testing apparatus and a magnetic field is impressed upon the separation zone, the free species of a labeled reagent is immobilized to the zone and the magnetically responsive reagent particles held by the reagent are held in the zone by magnetic forces and prevented from moving from the separation zone. Since a porous solid matrix used as a chromatography medium does not hinder the movement of the binding species of the labeled reagent to the matrix, the binding species is moved to a detection zone from the separation zone by a liquid testing medium. Then the detectable chemical group of the binding species in the detection zone is measured. In addition, when the separation of the binding species and an immobilized free species from each other starts, a detectable signal is generated in the detection zone without performing any operating process, etc. Therefore, the binding species of the labeled reagent can be separated and detected in a short time under control. |
