| 摘要 |
<p>A desired immunoglobulin, enzyme inhibitor, lectin, nucleic acid or nucleic acid derivative is separated from a protein complex through adsorption on a polymer carrier such as a polyhydric organic polymer, silicon gel, organic polyamine or polyamide or the like, into which have been introduced ligands containing an aliphatic group consisting of a thio-ether and a sulphone group or a thio-ether and an aromatic group or a thio-ether and a heterocyclic nitrogen or sulphur compound, or that the ligand instead of the thio-ether contains an amine or a sulphone group in combination with an aromatic or heterocyclic nitrogen or sulphur compound. The adsorption takes place in a salt environment, using antichaotropic salts such as sulphate, phosphate, chloride and the salts of polybasic acids, e.g. citrate, which promote adsorption at mol concentrations exceeding 1/5 of their solubility in water. Low-molecular substances in complex with proteins can be separated in accordance with the invention, but it is preferably used to separate protein-protein complexes in which both components have a molecular weight exceeding 10,000. However, the invention can also be applied to molecular weights as low as >100. The separation is preferably performed in a pH interval not exceeding 3 pH units from the isoelectric point of the complex. The barrier may be in the form of a thin layer, such as individual fibres, weave or paper, or it may be in the form of a solution in order to permit rapid adsorption, in which case it is precipitated out after the adsorption.</p> |
