| 摘要 |
PURPOSE: To determine the presence of nerve growth factor receptor-carrying tumor by detecting increase of nerve growth factor receptor fragment level through crosslinking/immunoprecipitation assay. CONSTITUTION: A purified nerve growth factor(NGF) is labeled with I-125. The I-125 labeled NGF and a sample containing nerve growth factor receptor fragment(NGFRt) are incubated. The I-125 labeled NGF bonded receptor is then crosslinked with 1-ethyl-3--(dimethylaminopropyl) carbodiimide. The crosslinked receptor is subjected to immunoprecipitation by an anti-NGFR monoclonal antibody. Subsequently, supernatant is subjected to dodecyl sodium sulfate-polyacrylamide gel electrophoresis and visualized by autoradiography. According to the method, NGFRt can be detected and presence of nerve growth factor receptor carrying tumor can be determined.
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