PROCESS FOR DIAGNOSIS AGENT OF HEPATITIS-B VIRUS

摘要

A methods for conjugating horseradish peroxidase(HRP) to the surface antigen of hepatitis B virus (HBsAg) comprises (a) combining the (subtype adw-and ady-) HBsAg and 4-(N-maleiimidomethyl cyclohexane-1- carboxylic acid-N-hydroxy succinimide ester (SMCC) to form 4-(N- maleiimidomethyl)-cyclohexane-1-carboxylic-HBsAg (I); (b) reacting HRP with the N-succinimidyl-3-)2-pyridyldithio) propionate (SPDP) and reducing by use of dithiothreitol to form -SH3-(2-pyridyldithio) propionyl-HRP(II); (c) combining (I) and (II) to form covalently bonded HRP-HBsAg conjugate.