发明名称 |
FORMING METHOD OF AUTOPOLYPLOID OF FUNGI |
摘要 |
PURPOSE:To obtain the subject polyploid for producing industrially useful genetic recombinant at high frequency and in a short time without using genetic marker by exposing test cell of conidia or mycelium of fungi to colchicines having polyploid-organization potency in cell division period. CONSTITUTION:Test cell (e.g. Trichoderma reesei QM 9414 strain) of conidia or mycelium of fungi is subjected to shake culture in liquid medium containing colchicine or/and colchicine derivative having polyploid-organization potency (e.g. colcemid) in cell division period of said cell at a temperature of 30 deg.C for 2-3 day and the cell formed many pellet-like aggregates is transplanted on agar-agar medium, then cultured at a temperature of 30 deg.C for 2-3 day to afford the aimed autopolyploid of fungi having increased nuclear size. |
申请公布号 |
JPH02231080(A) |
申请公布日期 |
1990.09.13 |
申请号 |
JP19890050091 |
申请日期 |
1989.03.03 |
申请人 |
YAKULT HONSHA CO LTD |
发明人 |
IMAI TOYOHIKO;TOYAMA NOBUO |
分类号 |
C12N15/09;C12N1/15;C12N15/01;C12R1/66;C12R1/845;C12R1/885 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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