| 摘要 |
The present invention provides a method for the determination of a compound having mercapto group which comprises reacting the compound with a chromogen represented by the following general formula (I): <IMAGE> (I) wherein Y is hydrogen or hydroxyl; R1, R2 and R3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): <IMAGE> (II) <IMAGE> (III) <IMAGE> (IV) wherein Z (which may be the same or different) is hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 0, 1, 2, or 3; provided that at least one Z in R1, R2 and R3 is hydroxyl, amino, or substituted amino, in the presence of peroxidase or thiol oxide reductase, and determining the pigment thus formed.
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