发明名称 |
METHOD OF REMOVING N-TERMINAL AMINO ACID RESIDUES FROM EUCARYOTIC POLYPEPTIDE ANALOGS AND POLYPEPTIDES PRODUCED THEREBY |
摘要 |
The invention relates to a method of producing a human growth hormone having the amino acid sequence of naturally occurring human growth hormone which comprises a) producing in a microbial host a first polypeptide which is characterized by the presence of one or more additional amino acids at the N-terminus of the amino acid sequence of naturally occurring human growth hormone; b) contacting the first polypeptide so produced with an enzyme, preferably an aminopeptidase, so as to produce a second polypeptide having the amino acid sequence of naturally occurring human growth hormone; and c) recovering the second polypeptide so produced. A preferred microbial host is a bacterium, especially E. coli. |
申请公布号 |
IL76107(A) |
申请公布日期 |
1991.03.10 |
申请号 |
IL19850076107 |
申请日期 |
1985.08.15 |
申请人 |
BIO-TECHNOLOGY GENERAL CORP. |
发明人 |
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分类号 |
C12N9/48;A61K35/74;A61K38/27;C07K1/22;C07K14/00;C07K14/195;C07K14/41;C07K14/52;C07K14/555;C07K14/575;C07K14/61;C07K14/65;C12N15/18;C12N15/20;C12N15/23;C12P21/00;C12P21/06;C12R1/01;C12R1/07;C12R1/545 |
主分类号 |
C12N9/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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