Glycosaminoglycans

摘要

Heparan sulphate HS/BMP2 is disclosed, together with the use of HS/BMP2 in the repair and regeneration of bone tissue.

主权项

1. A method of treatment of injury or disease, the method comprising administering, to an individual in need thereof, a therapeutically effective amount of an isolated or substantially purified HS/BMP2, wherein HS/BMP2 is heparan sulphate which is capable of specific binding to SEQ ID NO:1 or 6, and to BMP2, and which is obtainable by a method comprising:
(i) providing a solid support having polypeptide molecules adhered to the support, wherein the polypeptide consists of the amino acid sequence QAKHKQRKRLKSSCKRHP [SEQ ID NO:1] or QAKHKQRKRLKSSCKRH [SEQ ID NO:6]; (ii) contacting the polypeptide molecules with a heparan sulphate fraction obtained from mammalian tissue or extracellular matrix such that polypeptide-heparan sulphate complexes are allowed to form; (iii) partitioning polypeptide-heparan sulphate complexes from the remainder of the mixture; (iv) dissociating the heparan sulphate from the polypeptide-heparan sulphate complexes by disrupting the polypeptide-heparan sulphate complexes; and (v) collecting the dissociated heparan sulphate; wherein the method comprises the repair and/or regeneration of bone.