| 摘要 |
PURPOSE:To obtain a crude drug extract for the oral cavity useful for preventing periodontosis by extracting a crude drug selected from Psoraleae Semen, Pyrola japonica Klenze, Curcumae Rhizoma, Akebiae Caulis, Euphorbie Lathyridis Semen, Benincasae Semen and Pruni Cortex with methanol, ethanol and water. CONSTITUTION:Psoraleae Semen, Pyrola japonica Klenze, Curcumae Rhizoma, Akebiae Caulis, Euphorbie Lathyridis Semen, Benincasae Semen and Pruni Cortex are used as a crude drug and extracted with methanol and then ethanol, further water as extracting solvents at ambient temperature to 55 deg.C. The resultant extracts are then mixed and the methanol and ethanol are distilled away. The residue is subsequently fractionated with ethyl acetate and water by liquid- liquid partition. Furthermore, the obtained water fraction is extracted and fractionated with butanol and the ethyl acetate fraction is extracted and fractionated with hexane and methanol. Thereby, the objective crude drug extract is obtained. The resultant extract has selective antimicrobial activity against causative bacteria of periodontosis with low danger for microbial substitution even in prolonged administration for a long period. |
