| 摘要 |
PCT No. PCT/GB87/00663 Sec. 371 Date Apr. 13, 1989 Sec. 102(e) Date Apr. 13, 1989 PCT Filed Sep. 22, 1987 PCT Pub. No. WO88/02489 PCT Pub. Date Apr. 7, 1988.A biological substance is determined with the use of a marker comprising a lanthanide or other metal ion coupled to the biological substance by means of chelate-forming compound. The chelate complex is dissociated by reduction of the ambient pH to a sufficiently low level to liberate the metal ion and the metal ion is then chelated with a separate chelating chromophore with an increase in the pH to a value closer to the optimum for determination of the metal-chelated chromophore by fluorescence spectroscopy, luminometry, colourometry or the like.
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