BIOLUMINESCENCE ASSAY FOR GENE EXPRESSION IN GROWING MAMMALIAN CELLS

摘要

A method for detecting the presence of a certain substance in a medium is provided which is based on the use of genetically engineered tester cells which are capable of absorbing and of expressing the luciferase enzyme in the presence of said substance in their surrounding medium. In the performance of this method, cells are incubated with a tested medium and light generation under appropriate conditions is monitored, light generation above background levels being an indication of the presence of said substance in the tested medium. 82102.IC:lk/rrb