| 摘要 |
PURPOSE:To industrially and inexpensively obtain the subject compound by culturing a variant belonging to the genus Brevibacterium or Corynebacterium, capable of producing L-glutamic acid, and having resistance to antibiotics with inhibitory action on cell wall synthesis. CONSTITUTION:A variant [e.g.Brevibacterium lactofermentum-vancomcin resistant variant AJ12,557 (FERM P-11,730)] belonging to the genus Brevibacterium or Corynebacterium, capable of producing L-glutamic acid, and having resistance to antibiotics with inhibitory action on cell wall synthesis, is inoculated into a medium having an osmotic pressure of 2,000-4,000 mOSm/kg.H2O which is higher than a common medium in L-glutamic acid fermentation, subjected to shaking culture at 31.5 deg.C, L-glutamic acid is formed and accumulated in the culture solution and collected to give the objective L-glutamic acid. |
