| 摘要 |
<p>PURPOSE:To produce a cultured cancer tissue capable of sensitively measuring the effect of an anticancer agent in chemotherapy or radiotherapy, thermotherapy, etc., in physiotherapy on the cancer tissue in the living body in vitro by allowing cancer cells and normal cells to coexist and giving a duct structure to the cancer tissue. CONSTITUTION:Normal cells are cultured on a culture medium containing a temperature-sensitive polymer at a temperature of >=LCST so as to cover the surface of the culture medium with the normal cells. Thereby, a normal cell sheet is formed and cancer cells are inoculated and bonded to the normal cell sheet. The temperature is subsequently lowered to <=LCST and the normal cell sheet containing the cancer cells bonded thereto is released from the surface of the culture medium and allowed to float in the culture solution. This floating cell sheet is cultured on a cell-nonadhesive culture medium, thus producing the objective cultured cancer tissue. LCST is preferably 0 to 80 deg.C, further preferably 10 to 45 deg.C.</p> |
