| 摘要 |
PURPOSE:To efficiently produce a polysaccharide useful as a food additive, a base material of a medicine, etc., with a high productivity while reducing the cost by fixing a fine alga secreting the polysaccharide outside the body to the surface of a luminous material, culturing it and collecting the product. CONSTITUTION:A fine alga secreting a polysaccharide outside the body, e.g. a blue- green algae Aphanocapsa halophytia is cultured till the stationary phase in a culture solution prepared by adding Na2HPO4, NaNO3, etc., as a nutritive salt to a sterilized natural sea water. After collecting the cultured algae by centrifugation, the concentrated algae are suspended in a buffer solution and 4wt.% sodium alginate solution is mixed therewith in a volume ratio of 1:1. A luminous material, e.g. an optical fiber from which the clad layer is removed is immersed in the mixture solution and taken out therefrom after about 10sec. Immediately after take-out, the luminous material is immersed in 0.5N CaCl2 solution and allowed to stand for about 1hr. Thereby, the fine algae are buried in a calcium alginate gel and fixed to the surface of the luminous material. The fixed algae are subsequently put in a culture solution and cultured for 6 day according to the aeration agitation method while supplying light to the optical fiber. The culturced solution is desalted by dialysis, thus efficiently producing the objective polysaccharide. |
